Publications

Peer-reviewed papers10 papers

1. Neutrophil–Macrophage Interactions Shape Inflammatory Macrophage Remodeling in Gastric Cancer During Chemo–Immunotherapy
   Nagaoka KH, Yamashita H, Mashima C, Zhang G, Saito H, Minamide T, Nakatsuru T, Kamata R, Sakai SA, Yamamoto G, Morita TY, Nakai K, Hakozaki Y, Kinoshita K, Takahashi Y, Nakayama I, Kawazoe A, Pulukuri SM, Ishii Y, Haeno H, Kashima Y, Shitara K, Yano T, Saito T, Ohashi A
   Cancer Science, 117(8):2115-2131 (2026)doi: 10.1111/cas.70384
2. A cDC1–NK/T–Malignant Cell Circuit Drives Immunogenic Remodeling Under PD-1 Blockade in Gastric Cancer
   Nakatsuru T, Yamashita H, Mashima C, Zhang G, Saito H, Minamide T, Nagaoka KH, Kamata R, Sakai SA, Yamamoto G, Morita TY, Nakai K, Hakozaki Y, Kinoshita K, Takahashi Y, Nakayama I, Kawazoe A, Ishii Y, Pulukuri SM, Haeno H, Kashima Y, Shitara K, Yano T, Saito T, Ohashi A
   Cancer Science, 117(8):2098-2114 (2026)doi: 10.1111/cas.70381
3. Tumor-Agnostic Circulating Tumor DNA Analysis as a Comprehensive Predictive Biomarker of Recurrence after Ablative Radiotherapy for Early-Stage Non-Small Cell Lung Cancer
   Nakamura M, Kageyama SI, Hirata H, Ohira M, Sakai SA, Hakozaki Y, Hojo H, Fujisawa T, Yamashita R, Haeno H, Kanai A, Suzuki Y, Yamaguchi M, Tsuchihara K, Zenda S
   Clinical Lung Cancer, 26(8):671-679 (2025)doi: 10.1016/j.cllc.2025.09.013
4. Single-cell spatial analysis with Xenium reveals anti-tumour responses of CXCL13+ T and CXCL9+ cells after radiotherapy combined with anti-PD-L1 therapy
   Sakai SA, Oyoshi H, Nakamura M, Taki T, Nomura K, Hojo H, Hirata H, Motegi A, Nakamura Y, Zenkoh J, Aokage K, Hamada A, Kojima M, Kuwata T, Tsuchihara K, Akimoto T, Soh J, Mitsudomi T, Tsuboi M, Ishii G, Suzuki Y, Suzuki A, Yamashita R, Kageyama SI
   British Journal of Cancer, 133(6):795-808 (2025)First authordoi: 10.1038/s41416-025-03088-0
5. Microbiome landscape and association with response to immune checkpoint inhibitors in advanced solid tumors: a SCRUM-Japan MONSTAR-SCREEN study
   Sawada K*, Yamashita R*, Sakai SA*, Horasawa S, Yoshikawa A, Fujisawa T, Kadowaki S, Kato K, Ueno M, Oki E, Komatsu Y, Chiyoda T, Horita Y, Yasui H, Denda T, Satake H, Esaki T, Satoh T, Takahashi N, Yamazaki K, Matsuhashi N, Nishina T, Takeda H, Ohtsubo K, Ohta T, Tsuji A, Goto M, Kato T, Bando H, Tsuchihara K, Nakamura Y, Yoshino T (*equal contribution)
   Cancer Research Communications, 5(5):857-870 (2025)Co-first authordoi: 10.1158/2767-9764.CRC-24-0543
6. SpatialKNifeY (SKNY): Extending from spatial domain to surrounding area to identify microenvironment features with single-cell spatial omics data
   Sakai SA, Nomura R, Nagasawa S, Chi S, Suzuki A, Suzuki Y, Imai M, Nakamura Y, Yoshino T, Ishikawa S, Tsuchihara K, Kageyama S, Yamashita R
   PLOS Computational Biology, 21(2):e1012854 (2025)First authordoi: 10.1371/journal.pcbi.1012854
7. Mathematical Modeling Predicts Optimal Immune Checkpoint Inhibitor and Radiotherapy Combinations and Timing of Administration
   Sakai SA, Saeki K, Chi SG, Hamaya Y, Du J, Nakamura M, Hojo H, Kojima T, Nakamura Y, Bando H, Kojima M, Suzuki A, Suzuki Y, Akimoto T, Tsuchihara K, Haeno H, Yamashita R, Kageyama S-I
   Cancer Immunology Research, 13(3):353-364 (2025)First authordoi: 10.1158/2326-6066.CIR-24-0610
8. Gut microbiome associated with PARP inhibitor efficacy in patients with ovarian cancer
   Okazawa-Sakai M*, Sakai SA*, Hyodo I, Horasawa S, Sawada K, Fujisawa T, Yamamoto Y, Boku S, Hayasaki Y, Isobe M, Shintani D, Hasegawa K, Egawa-Takata T, Ito K, Ihira K, Watari H, Takehara K, Yagi H, Kato K, Chiyoda T, Harano K, Nakamura Y, Yamashita R, Yoshino T, Aoki D (*equal contribution)
   Journal of Gynecologic Oncology, 36(3):e38 (2025)Co-first authordoi: 10.3802/jgo.2025.36.e38
9. Comprehensive single-cell analysis demonstrates radiotherapy-induced infiltration of macrophages expressing immunosuppressive genes into tumor in esophageal squamous cell carcinoma
   Oyoshi H, Du J, Sakai SA, Yamashita R, Okumura M, Motegi A, Hojo H, Nakamura M, Hirata H, Sunakawa H, Kotani D, Yano T, Kojima T, Nakamura Y, Kojima M, Suzuki A, Zenkoh J, Tsuchihara K, Akimoto T, Shibata A, Suzuki Y, Kageyama SI
   Science Advances, 9(50):eadh9069 (2023)doi: 10.1126/sciadv.adh9069
10. Fecal microbiota in patients with a stoma decreases anaerobic bacteria and alters taxonomic and functional diversities
    Sakai SA, Aoshima M, Sawada K, Horasawa S, Yoshikawa A, Fujisawa T, Kadowaki S, Denda T, Matsuhashi N, Yasui H, Goto M, Yamazaki K, Komatsu Y, Nakanishi R, Nakamura Y, Bando H, Hamaya Y, Kageyama S-I, Yoshino T, Tsuchihara K, Yamashita R
    Frontiers in Cellular and Infection Microbiology, 12:925444 (2022)First authordoi: 10.3389/fcimb.2022.925444

Preprints

1. Distinct Gut Microbiome Landscape of PIK3CA-Mutated Colorectal Cancer: A Nationwide Pan-Cancer Genomic and Microbial Profiling Study (MONSTAR-SCREEN)
   Boku S, Sakai S, Sawada K, Horasawa S, Yoshikawa A, Nakamura Y, Fujisawa T, Yamashita R, Satake H, Komatsu Y, Nishina T, Shiozawa M, Kudo T, Matsuhashi N, Yamazaki K, Yamaguchi T, Yasui H, Takahashi N, Kadowaki S, Denda T, Yoshino T
   Research Square (2026)doi: 10.21203/rs.3.rs-9627410/v1
2. Development of a Deep Learning Model Integrating CT Images and Blood Data for the Diagnosis of Acute Cholecystitis
   Horaguchi T, Nomura R, Sakai SA, Saito N, Kurihara K, Ohira M, Takaha R, Mitsui N, Hatanaka Y, Yokoi R, Kuno M, Hayashi H, Fukada M, Sato Y, Yasufuku I, Asai R, Bando H, Yamashita R, Matsuhashi N
   medRxiv (2026)doi: 10.64898/2026.05.08.26352724
3. Catalog of gut microbiota alterations associated with anticancer therapies across multiple cancer types
   Kurihara K, Sakai SA, Sawada K, Iida N, Horasawa S, Fujisawa T, Nakamura Y, Kageyama SI, Bando H, Yoshino T, Tsuchihara K, Yamashita R
   bioRxiv (2026)doi: 10.64898/2026.05.08.723707
4. Single-cell spatial multiomics identifies POSTN+ CAFs mediating chemoradiotherapy resistance in rectal cancer
   Sakai SA, Okumura M, Morinaga Y, Kato K, Kojima M, Hofmann F, Reichholf B, Garcia PV, Nakamura Y, Sakashita S, Nakamura M, Hojo H, Tsukada Y, Ito M, Shitara K, Bando H, Kojima T, Zenkoh J, Tatekawa S, Katsuki S, Ogawa K, Takahashi Y, Suzuki A, Suzuki Y, Tsuchihara K, Gremel G, Yamashita R, Kageyama SI
   bioRxiv (2026)First authordoi: 10.64898/2026.04.30.721803
5. SpatialCompassV (SCOMV): De novo cell and gene spatial pattern classification and spatially differential gene identification
   Nomura R, Sakai SA, Kageyama SI, Tsuchihara K, Yamashita R
   bioRxiv (2026)doi: 10.64898/2026.02.26.707418

Japanese articles和文総説・解説

1. 腫瘍内科診療におけるAI/Radiomicsの意義
   影山 俊一郎, 酒井 俊輔, 野村 亮輔, 藤澤 孝夫, 坂東 英明, 山下 理宇
   腫瘍内科, 37(6):613-618 (2026) · 科学評論社CiNii
2. 空間トランスクリプトーム解析 (Spatial Transcriptomics: ST) による創薬
   影山 俊一郎, 酒井 俊輔, 藤澤 孝夫, 山下 理宇
   BIO Clinica, 41(7):611-616 (2026) · 北隆館CiNii

Conference abstractspublished

• Kamiya H, Sakai SA, Kageyama SI, Yamashita R, Katoh H, Kojima M, Suzuki Y, Kudo M, Sugimoto M, Kobayashi S, Gotohda N. (2026) Single-Cell Spatial Analysis Reveals the Process of dedifferentiation in Undifferentiated Pancreatic Cancer. Laboratory Investigation, 106(3), 1515.
• Nomura R, Sakai SA, Yamashita R, Kageyama SI, Tsuchihara K. (2026) Unsupervised annotation of spatial transcriptomes based on vectorial information between transcripts. Cancer Science, 117:712.
• Kamiya H, Sakai SA, Kageyama SI, Yamashita R, Katoh H, Kojima M, Suzuki Y, Kudo M, Sugimoto M, Kobayashi S, Gotohda N. (2026) Single-Cell Spatial Analysis Reveals the Process of Sarcomatous change in Undifferentiated Pancreatic Cancer. Cancer Science, 117:286.
• Sakai SA, Nomura R, Kojima M, Suzuki A, Suzuki Y, Tsuchihara K, Kageyama SI, Yamashita R. (2026) Application of SpatialKNifeY to Spatial Transcriptomics for Extracting Tissue Structure and Cataloging Microenvironment. Cancer Science, 117:1024.
• Kurihara K, Sakai SA, Iida N, Sawada K, Horasawa S, Fujisawa T, Nakamura Y, Kageyama SI, Tsuchihara K, Yamashita R. (2026) Development of a Catalog of Anticancer Drug Effects on the Human Gut Microbiome Across Cancer Types. Cancer Science, 117:781.
• Sakai SA, Nomura R, Nagasawa S, Chi S, Suzuki A, Suzuki Y, Tsuchihara K, Kageyama SI, Yamashita R. (2025) Molecular analysis for the clarification of carcinogenesis. Cancer Science, 116:393.
• Kageyama SI, Sakai SA, Saeki K, Haeno H, Hojo H, Nakamura M, Akimoto T, Suzuki A, Suzuki Y, Yamashita R, Tsuchihara K. (2025) Identification of Tumor Microenvironment Changes Post-Radiotherapy via Single-Cell and Spatial Transcriptome. Cancer Science, 116:176.
• Harima T, Yamashita R, Nakamura Y, Sakai S, Nomura R, et al. (2024) 555P Association between copy number aberration and ctDNA MRD in colorectal cancer: CIRCULATE-Japan GALAXY. Annals of Oncology, 35:S458.
• Oyoshi H, Du J, Sakai SA, Yamashita R, Okumura M, Nakamura M, Hirata H, Sunakawa H, Yano T, Kojima T, Kojima M, Tsuchihara K, Akimoto T, Shibata A, Suzuki Y, Kageyama SI. (2024) Temporal single-cell analysis reveals time-dependent immunological modulation by radiotherapy in esophageal cancer. Cancer Science, 115:1972.
• Boku S, Sakai S, Sawada K, Horasawa S, Yoshikawa A, Nakamura Y, et al. (2023) MO32-5 Metabolic interaction between gut microbiome and PIK3CA-mutated colorectal cancer: SCRUM-Japan MONSTAR-SCREEN. Annals of Oncology, 34:S1416.
• Matsubara N, Sakai S, Yamashita R, Misumi T, Shiota M, Eto M, Kato T, et al. (2023) The comprehensive analysis of relationship between gut microbiome and treatment outcome of androgen deprivation therapy (ADT)-based treatment in patients with metastatic prostate cancer. Journal of Clinical Oncology, 41(6_suppl):213.
• Tezuka S, Sakai S, Yamashita R, Horasawa S, Fujisawa T, Sawada K, et al. (2023) Interim analysis results of gut microbiota in patients with unresectable cholangiopancreatic cancer: SCRUM-Japan MONSTAR-SCREEN. Journal of Clinical Oncology, 41(4_suppl):733.
• Sakai SA, Nakamura Y, Bando H, Kageyama SI, Yoshino T, Tsuchihara K, Yamashita R. (2023) Fecal microbiota in patients with a stoma decreases anaerobic microbes and alters various diversities. Cancer Science, 114:330.

Oral presentations15

Short talks6

Poster presentations14

Organized sessions4